{"id":1303,"date":"2026-01-24T08:35:20","date_gmt":"2026-01-24T08:35:20","guid":{"rendered":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/2026\/01\/24\/modulation-of-iodide-uptake-and-retinoic-acid-receptors-rar-and-rxr-expression-by-thyrotropin-and-re\/"},"modified":"2026-01-24T08:35:20","modified_gmt":"2026-01-24T08:35:20","slug":"modulation-of-iodide-uptake-and-retinoic-acid-receptors-rar-and-rxr-expression-by-thyrotropin-and-re","status":"publish","type":"post","link":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/2026\/01\/24\/modulation-of-iodide-uptake-and-retinoic-acid-receptors-rar-and-rxr-expression-by-thyrotropin-and-re\/","title":{"rendered":"MODULATION OF IODIDE UPTAKE AND RETINOIC ACID RECEPTORS (RAR AND RXR) EXPRESSION BY THYROTROPIN AND RETINOIC ACID"},"content":{"rendered":"<div class=\"article-authors\">Souza CL.E.<sup>1<\/sup>, Coelho M.S.<sup>2<\/sup>, Alves M.A.<sup>2<\/sup>, Takyia M.Ch.<sup>2<\/sup>, Nasciutti L.E.<sup>2<\/sup>, Carvalho P.D.<sup>1<\/sup><\/div>\n<div class=\"article-institutes\"><sup>1<\/sup>Instituto de Biofisica Carlos Chagas Filho, UFRJ, <sup>2<\/sup>Departamento de Histologia e Embriologia, UFRJ<\/div>\n<div class=\"article-abstract\">\n<h2>Abstract<\/h2>\n<p>etinoic acid (RA) has been used to reinduce iodide uptake in advanced thyroid cancer, however retinoic acid receptors regulation in thyroid cells has not been well defined. We aimed to determine the mechanisms by which RA acts on the sodium-iodide symporter (NIS) function and the regulation of retinoic acid receptors RAR (a, b e g) and RXR (a, b e g) expression in the normal thyroid cell line PCCL3. PCCL3 cells were treated during 72 hours with all-trans-RA (1mM), 13-cis-RA (1mM) or vehicle (ethanol 0.1%), in the presence or absence of TSH. Retinoic acid did not change cellular viability. RA treatment significantly decreased iodide uptake by PCCL3 and NIS mRNA expression. NIS protein is still present in 72h-RA treated cells due its long half-life; however RA treatment decreased the presence of NIS in the plasma membrane, as assessed by confocal microscopy. Expression and cellular localization of the receptors was evaluated by immunofluorescence. RARa, RXRa, RXRb and RXRg receptors were expressed and localized in the nucleus, while RARb was localized in the cytoplasm. RARg receptor expression was not detected in these cells. RA treatment and the absence of TSH for 48 h both promoted a downregulation of RXRg expression. When TSH is replaced for 48h iodide uptake is restored, without normalization of RXRg expression. <br \/>In conclusion, PCCL3 cells express almost all isoforms of RA receptors, except RARg, at least in the presence of TSH. RA treatment decrease iodide uptake even when in the presence of RARs and the absence of RXRg. Financial Support: CNPq, CAPES, FAPERJ. <\/div>\n<div class=\"article-content\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Souza CL.E.1, Coelho M.S.2, Alves M.A.2, Takyia M.Ch.2, Nasciutti L.E.2, Carvalho P.D.1 1Instituto de Biofisica Carlos Chagas Filho, UFRJ, 2Departamento de Histologia e Embriologia, UFRJ Abstract etinoic acid (RA) has been used to reinduce iodide uptake in advanced thyroid cancer, however retinoic acid receptors regulation in thyroid cells has not been well defined. We aimed <a class=\"read-more\" href=\"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/2026\/01\/24\/modulation-of-iodide-uptake-and-retinoic-acid-receptors-rar-and-rxr-expression-by-thyrotropin-and-re\/\">Read More<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[45,2,9],"tags":[],"class_list":["post-1303","post","type-post","status-publish","format-standard","hentry","category-volume-7-issue-3","category-journal-articles","category-volume-7"],"_links":{"self":[{"href":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/wp-json\/wp\/v2\/posts\/1303","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/wp-json\/wp\/v2\/comments?post=1303"}],"version-history":[{"count":0,"href":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/wp-json\/wp\/v2\/posts\/1303\/revisions"}],"wp:attachment":[{"href":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/wp-json\/wp\/v2\/media?parent=1303"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/wp-json\/wp\/v2\/categories?post=1303"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/wp-json\/wp\/v2\/tags?post=1303"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}