{"id":1249,"date":"2026-01-24T08:35:20","date_gmt":"2026-01-24T08:35:20","guid":{"rendered":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/2026\/01\/24\/in-situ-detection-of-micrornas-146b-221-and-222-in-formalin-fixed-paraffin-embedded-human-carcinoma\/"},"modified":"2026-01-24T08:35:20","modified_gmt":"2026-01-24T08:35:20","slug":"in-situ-detection-of-micrornas-146b-221-and-222-in-formalin-fixed-paraffin-embedded-human-carcinoma","status":"publish","type":"post","link":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/2026\/01\/24\/in-situ-detection-of-micrornas-146b-221-and-222-in-formalin-fixed-paraffin-embedded-human-carcinoma\/","title":{"rendered":"IN SITU DETECTION OF MICRORNAS 146B, 221 AND 222 IN FORMALIN-FIXED, PARAFFIN-EMBEDDED HUMAN CARCINOMA TISSUES REVEALS SPECIFIC EXPRESSION PATTERNS IN THYROID CARCINOMAS"},"content":{"rendered":"<div class=\"article-authors\">Kotoula V.<sup>1<\/sup>, Charalambous E.<sup>1<\/sup>, Koletsa T.<sup>1<\/sup>, Pliakos I.<sup>1<\/sup>, Karayannopoulou G.<sup>1<\/sup>, Karkavelas G.<sup>1<\/sup>, Mitsiades S.C.<sup>2<\/sup>, Mitsiades N.<sup>2, 3<\/sup><\/div>\n<div class=\"article-institutes\"><sup>1<\/sup>Dept. of Pathology, School of Medicine, Aristotle University, Thessaloniki, Greece, <sup>2<\/sup>Dept. of Medical Oncology, Dana Farber Cancer Institute, and Dept. of Medicine, Harvard Medical School, Boston MA, USA, <sup>3<\/sup>Memorial Sloan Kettering Cancer Center, New York, USA<\/div>\n<div class=\"article-abstract\">\n<h2>Abstract<\/h2>\n<p><strong>Context:<\/strong> MicroRNAs (miRs) are transcribed from non-protein-encoding genes and regulate the post-transcriptional fate of protein-encoding mRNAs. Over-expression of miR146b, miR221 and miR222 has been reported in papillary thyroid carcinomas (PTCs) with methods performed on tissue extracts.<br \/><strong>Objective:<\/strong> To determine the expression of miR146b, 221 and 222 in situ in thyroid carcinomas using routine formalin-fixed, paraffin-embedded (FFPE) tissue sections.<br \/><strong>Materials and Methods:<\/strong> Digoxigenin-labeled oligonucleotide DNA probes were designed to detect the mature and adjacent sequences of miRs146b, 221 and 222. In situ hybridization (ISH) was applied on 133 archival FFPE tissues including 105 thyroid carcinomas and a control group of tumors (n=28). Real time quantitative (RTQ)-PCR for the above miRs was used to validate the ISH results on the same thyroid carcinomas and in a thyroid carcinoma cell line panel.<br \/><strong>Results:<\/strong> Intense cytoplasmic ISH signals in &gt;10% of tumor cells for the individual miRs (strong positivity) were validated with RTQ-PCR as reflecting over-expression of the corresponding mature miR sequence. Triple miR146b\/221\/222 strongly positive profiles were observed more frequently in PTC than in medullary (MTC) or anaplastic carcinomas (p&lt;0.0001), while triple negative results were obtained in 4\/4 PTCs with very aggressive behaviour, in 3\/3 follicular, in 13\/14 anaplastic carcinomas, and in 11\/13 cell lines. Single-cell positivity was encountered in carcinomas but also in non-cancerous epithelia. Nuclear ISH positivity was characteristic of MTCs.<br \/><strong>Conclusions:<\/strong> Specific miR patterns may be useful in the diagnostic setting. Down-regulation of miRs146b, 221 and 222 in thyroid carcinoma may be related to aggressive behavior in PTCs and ATCs.<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Kotoula V.1, Charalambous E.1, Koletsa T.1, Pliakos I.1, Karayannopoulou G.1, Karkavelas G.1, Mitsiades S.C.2, Mitsiades N.2, 3 1Dept. of Pathology, School of Medicine, Aristotle University, Thessaloniki, Greece, 2Dept. of Medical Oncology, Dana Farber Cancer Institute, and Dept. of Medicine, Harvard Medical School, Boston MA, USA, 3Memorial Sloan Kettering Cancer Center, New York, USA Abstract Context: <a class=\"read-more\" href=\"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/2026\/01\/24\/in-situ-detection-of-micrornas-146b-221-and-222-in-formalin-fixed-paraffin-embedded-human-carcinoma\/\">Read More<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[45,2,9],"tags":[],"class_list":["post-1249","post","type-post","status-publish","format-standard","hentry","category-volume-7-issue-3","category-journal-articles","category-volume-7"],"_links":{"self":[{"href":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/wp-json\/wp\/v2\/posts\/1249","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/wp-json\/wp\/v2\/comments?post=1249"}],"version-history":[{"count":0,"href":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/wp-json\/wp\/v2\/posts\/1249\/revisions"}],"wp:attachment":[{"href":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/wp-json\/wp\/v2\/media?parent=1249"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/wp-json\/wp\/v2\/categories?post=1249"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/peaceful-mccarthy.213-158-90-25.plesk.page\/index.php\/wp-json\/wp\/v2\/tags?post=1249"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}